摘要
对生菜子叶在含有不同激素浓度的培养基上进行了诱导、分化及成苗的研究,结果表明:在MS+0.5mg/LIAA+1.2mg/L6-BA的培养基上能够实现较高的不定芽再生,不定芽在1/2MS培养基上能健康成苗。同时以生菜子叶不同部位的切块为外植体,用MS+0.5mg/LIAA+1.2mg/L6-BA的培养基进行不定芽诱导试验,发现生菜子叶不同部位的切块在离体培养时存在着全息现象。
The factors that influence callus induction , shoot differentiation and growth of Lactuca sativa var. capitata L Cotyledon were studied and an efficient regeneration system was established. Shoot regeneration were better achieved from the medium of MS ＋ 0. 5mg·L^-1 IAA ＋1.2mg·L^-1 6-BA using different part of cotyledon explant. The shoots could grow up well from the medium of 1/2MS without addition of hormone, the holographic phenomenon can be seen using different part of cotyledon explant.
出处
《北方园艺》
CAS
北大核心
2007年第1期142-144,共3页
Northern Horticulture
关键词
生菜
子叶
组织培养
植物激素
全息现象
Lactuca sativa var. capitata L
Cotyledon
Tissue culture
Phytohormone
The holographic phenomenon
