亚硝基血红蛋白合成研究

Study on synthesis of HbNO

为获得猪血血红蛋白与亚硝酸钠反应合成亚硝基血红蛋白(HbNO)的最佳工艺条件,在单因素试验之上进行了正交试验。结果表明,最佳合成工艺参数为:pH值6.20,温度80℃,反应时间20min,亚硝酸钠添加量2.1%。产物进行紫外-可见分光光度扫描,鉴定其结构为亚硝基亚铁血色原色素(NFH),然后进行真空冷冻干燥。

Orthogonal experiment was made on the basis of single factor and level in order to attain the optimum parameters to synthesize FlbNO with porcine blood hemoglobin and NaNO2. The results showed that the optimum conditions were： NaNO2 2.1%, pH 6.2, time 20min, temperature 800C. Under above conditions, the product, which were dried by frozen up in vacuum, was nitrosyl ferrohemochome （NFH） with UV- visible spectroscopic analysis.