HPLC法同时测定银黄制剂中的绿原酸和黄芩苷含量

Simultaneous HPLC determination of chlorogenic acid and baicalin in Yinhuang preparations

目的:建立同时测定银黄制剂中绿原酸和黄芩苷的 HPLC 方法。方法:色谱柱为 Waters Symmetry C_(18)(3.9 mm×150mm,5μm)柱,以甲醇-0.4%磷酸水溶液为流动相,梯度洗脱(0→15 min,甲醇15%→55%;15→25 min,甲醇55%;25→28min,甲醇55%→15%)。流速为0.8 mL·min^(-1),检测波长为320 nm,0.02 AUFS。结果:绿原酸的线性范围为6.88～110.08μg·mL^(-1)(r=0.9996),平均回收率为97.4%,RSD 为2.2%(n:5);黄芩苷的线性范围为13.40～214.40μg·mL^(-1)(r=0.9998),平均回收率为96.7%,RSD 为2.0%(n=5)。结论:该方法简单、快速、准确,可为银黄制剂的质量控制提供实验依据。

Objective：To develop an RP - HPLC method for simultaneous determination of chlorogenic acid and baicalin in Yinhuang preparations. Methods：The chromatographic analysis were carried out on a Waters Symmetry C18 column（3.9 mm × 150 mm,5μm） with methanol-0. 4% phosphoric acid as mobile phase at 0. 8 mL·min^-1 flow rate in gradient mode （ 0→15 min ： methanol 15 % →55 %, 15→25 min ： methanol 55 %, 25→28 min ： methanol 55%→15% ）. Results：The linear ranges of chlorogenic acid and baicalin were from 6. 88 to 110. 08 μg·mL^-1 （r =0. 9996）and from 13.40 to 214. 40μg·mL^-1（r = 0. 9998 ） ,respectively. The mean recoveries of chlorogenic acid and baicalin were 97.4% with RSD 2. 2% （ a = 5 ） and 96. 7% with RSD 2. 0% （ a = 5 ）. Conclusion： The method is simple,fast and accurate. It can be used to control the quality of Chinese herb Yinhuang preparations.