不同培养条件对蝴蝶兰离体叶片不定芽发生的影响

Effects of different culture conditions on adventitious shoots regeneration from the excised leaves of Phalaenopsis

以蝴蝶兰品系RSW1离体叶片为外植体,探讨了基本培养基、6-苄基腺嘌呤(6-BA)和噻重氮苯基脲(TDZ)、暗培养时间及叶片生育期对叶片不定芽发生的影响。结果表明,基本培养基以MS、1/2MS最为适宜,改良Knudson C(改良KC)不利于不定芽发生。TDZ诱导不定芽发生的效果好于6-BA,1.5 mg.L-1TDZ对蝴蝶兰叶片不定芽诱导率最高,达到60.9%。适当时期的暗培养有利于不定芽的发生,以暗培养21 d效果最好,不定芽诱导率达到74.5%,分化系数达6.90。选择生育期45 d左右的叶片作外植体,可获得较高的不定芽诱导率和分化系数。

The excised leaf of Phalaenopsis RSWI was used as explants, the effects of basic media, kinds and concentrations of 6-benzyl aminopurine （6 -BA） and thidiazuron （TDZ）, dark culture phase, and leaf age on adventitious shoot regeneration from leaves of Phalaenopsis RSW1 were investigated. The result showed that MS and 1/2MS were the most suitable basic media, while improved Knudson C （KC） was disadvantageous to shoots regeneration. TDZ was more effective than 6 - BA in inducing shoot regeneration from leaves of Phalaenopsis RSW,. The highest regeneration rate of 60. 9% was obtained on the medium MS supplemented with 1.5 mg · L^- 1 TDZ. Appropriate dark treatment could increase the regeneration rate of adventitious shoot. Under the most effective condition of 21 days dark treatment, the induction rate of adventitious shoot reached to 74. 5% with the differentiation coefficient 6. 90. When those leaves aged 45 days was used as explants, higher induction rate and differentiation coefficient could be achieved.