未变性乳清蛋白对支气管哮喘儿童红细胞GSH和GSH-PX的影响

GSH and GSH-PX Effect of Undenatured Whey Protein on Asthma Children's Erythrocyte

目的:观察未变性乳清蛋白对支气管哮喘儿童GSH和GSH-PX的影响。方法:采集30例健康儿童血标本。收集56例哮喘患儿资料。所有病例随机分两组,治疗组30例采用未变性乳清蛋白加普米克(布地奈德气雾剂);对照组28例单用普米克治疗。分别测定健康儿童和支气管哮喘儿童对照组及治疗组红细胞浆内总谷胱甘肽(GSH)含量及谷胱甘肽过氧化物酶(GSH-PX)活性。结果:支气管哮喘儿童红细胞总GSH含量较健康儿童减少,GSH-PX活性降低;服用未变性乳清蛋白的治疗组较对照组红细胞GSH含量及GSH-PX活性有明显的提高。结论:未变性乳清蛋白能有效提高支气管哮喘患儿红细胞浆中GSH水平,增加GSH-PX活性。

Objective： To investigate glutathione （GSH）and activity of glutathione peroxidase（GSH-PX） effect of undenatured whey protein on asthma children＇s erythrocyte. Methods： Arterial blood sample were taken from 30 healthy children. 56 asthma children＇s information were collected and randomly divided into two groups： the treatment group had 30 cases which were given undenatured whey protein and Pulmicort（Budesonide Aerosol）,the control group had 28 cases which were given Pulmicort. Cord blood samples of healthy children and two groups of asthma children to determine GSH and GSH-PX in erythrocyte intracytoplasm. Results： Reduced glutathione and activity of glutathione peroxidase in asthma children were lower than that in healthy children,and the treatment group＇s were higher than the control group＇s. Conclusion： Reduced glutathione and activity of glutathione peroxidase with undenatured whey protein are ineeased.