不同剂量地塞米松对SIRS大鼠心肌酶CK-MB的影响

Effects of different doses of dexamethason on systemic inflammatory response syndrome rat's cardiac CK-MB

目的观察不同剂量地塞米松对SIRS大鼠心肌酶CK-MB的影响。方法Wistar大鼠40只，体重160～200g。雄性。随机分为5组：LPS注射后3h组（3．0h组）、LPS注射后6h组（6．0h组）、小剂量地塞米松治疗组（DXMI组）、大剂量地塞米松治疗组（DXMⅡ组）、正常对照组，每组8只。实验组大鼠给予大肠杆菌内毒素（E．Coli O55B5，美国Sigma公司产品）20mg／kg腹腔注射，制造SIRS大鼠模型，观察时间分别为3、6h，DXM组在同样给予LPS后10min给地塞米松，Ⅰ组给予10mg／kg，Ⅱ组给予20mg／kg腹腔注射，观察时间为3h，结果与LPS3．0h组进行对比，对照组腹腔注射等量无菌生理盐水。观察SIRS大鼠各个时间心肌酶谱CK-MB的变化。结果LPS3．0h组、LPS6．0h组分别与对照组比较，结果有显著性差异（P均〈0．01），尤以LPS6．0h组升高明显，说明SIRS大鼠心肌细胞受到严重损害。而且随着时间的推移，损害越来越重。与LPS3．0h组比较，DXMI组CK-MB值下降明显（P〈0．05），DXMⅡ组CK-MB值略有下降，但无显著性差异（P〉0．05）。结论SIRS大鼠心肌受到损害，大剂量地塞米松对SIRS大鼠心肌损害的改善不明显，小剂量地塞米松对SIRS大鼠心肌损害的疗效优于大剂量地塞米松。

Objective To explore the effects of different doses of dexamethason on systemic inflammatory response syndrome rat＇s cardiac CK-MB. Methods Forty Wistar rats were randomly divided into five groups： LPS 3.0 h grouD LPS 6.0 h group, DXM Ⅰ group, DXM Ⅱ group, control group. The SIRS model of rats was made by lipopolysaccharide （LPS） injection, and DXM Ⅰ group received DXM 10 mg/kg, DXM Ⅱ] group received DXM 20 mg/kg, intraperitoneally. The cardiac CK-MB of each group were observed and compared. Results Compared with the control group, the CK-MB of LPS 3.0 h group, LPS 6.0 h group were significantly increased （ all P 〈0.01 ）, Compared with LPS3.0 h group, the CK-MB of DXM I group significantly decreased（P〈 0.05）, but DXM I] group did not obviously decreased （P〈0.05）. Conclusion The effects of 10 mg/kg dose of dexamethason was better than 20 mg/kg dose of dexamethason on improvement of SIRS rat＇ s cardiac CK-MB.