樗白皮活性成分对结肠炎模型小鼠血清相关炎性因子的影响

Effect of Active Component of Ailanthus altissima on Correlative Phlogistic Factors in Serum of Model Mice of Ulcerative Colitis

以2,4-二硝基氯苯(DNCB)致小鼠结肠炎模型为研究对象,探讨了樗白皮活性成分对小鼠血清中NO含量和NOS活性的影响。小鼠分成4组(即对照组、预防组、治疗组、模型组)。模型组用DNCB-丙酮-橄榄油制剂先腹部涂抹致敏再进行DNCB-乙醇溶液灌肠;预防组和治疗组分别在制模前、后进行药物灌胃;对照组以乙醇灌肠和生理盐水灌胃。利用比色法对各组动物血清NO含量和NOS活性进行测定。结果,预防组与治疗组NO含量较模型组和对照组显著降低(p<0.001,p<0.01),预防组与治疗组间无差异显著性;预防组、治疗组NOS活性较模型组显著降低(p<0.01),但与对照组间无差异显著性。结论,樗白皮活性成分对机体NO和NOS具有调节作用,在结肠炎时能促进机体清除NO含量和下调NOS的活性,对于药物所致的小鼠结肠炎有缓解作用。

Effect of the extracts of Ailanthus altissima on NO and NOS in serum was investigated, setting the model of mice ulcerative colitis （UC） caused by 2,4-dinitrochlorobenzene （DNCB） as the subject. Mice were randomly divided into 4 groups ： control, prevention, treatment and model. In model group, mice were sensitized by smearing DNCB （3.3%, solvated in -olive oil ） on the abdominal skin, and then administrated intra-colonicaly by DNCB （0.04%, solvated in 60% ethanol）. In prevention group, mice were administrated intra-gastrically by extracts of Ailanthus altissima before being made as model; but in treatment group, they were done after being made as model. In control group, they were intra-colonicaly by 60% ethanol and administrated intra-gastrically by physiological saline. NO and NOS in serum were detected by spectyophotograph. As a result, the NO content in prevention and treatment groups was lower remarkably than that in control and model groups（P〈0.001 ,P 〈0.01 ）, and there was no difference between prevention and treatment groups. In prevention and treatment groups, the NOS content was lower remarkably than that in model group（P〈 0.01 ） ,but there was no difference with the control. It was concluded that the extracts of Ailanthus altissima had regulation effects on NO and NOS. It could clear up NO,reduce NOS activity＇, and relieve the mice ulcerative colitis caused bv medicine DNCB.