摘要
目的:建立参桂胶囊中阿魏酸的含量测定方法。方法:采用Lichrospher C18(4.6mm×250mm,5μm)色谱柱,以甲醇-1.5%冰醋酸溶液(50:51)为流动相,流速为1.01ml/min,柱温为25℃,检测波长为315nm。结果:阿魏酸在0.00408~0.0408μg范围内线性关系良好,γ=1.0000,平均回收率为102.26%。结论:本法灵敏准确,简便易行,重现性好。
Objective. To establish a method for the determination of ferulic acid in Shengui Capsules. Method: An ODS column was used with a mobile phase consisted of mixture of methanol 1.5 % Acetic acid at a flow rate of 1.0 ml/min in this method, and UV detector wavelength was 315 nm. Results: Ferulic acid had a good linearity in the range of 0. 00408-0. 0408μg and γ= 1. 0000;the average recovery rate was 102.26 %, Conclusion: This method is simple, accurate and with good reproducibility.
出处
《河南中医学院学报》
2007年第1期47-48,共2页
Journal of Henan University of Chinese Medicine