实验性脾虚证大鼠肝脏和肌肉组织中糖原变化的组织学观察

Changes of the level of glycogen in both liver and muscle in spleen deficiency syndrome rats

将45只大白鼠随机分成对照组、脾虚模型组及治疗组,每组15只。以利血平(1mg/kg·d)复制大白鼠脾虚证模型,四君子汤辅助治疗,在实验进行至第7天和第14天分别捕杀各组大白鼠,取肝脏、肌肉组织,用组织化学方法测定在不同组间和不同实验阶段其中糖原的变化。结果,在第7天时,脾虚组和治疗组糖原含量明显低于正常对照组,第14天时,与正常对照组相比较,脾虚组和治疗组糖原含量显著升高,且脾虚组糖原含量较其它两组高,提示肝脏和肌肉组织中糖原的变化可能是脾虚证发生发展的重要指标。

45 Wistar rats were divided into the control group, spleen deficiency syndrome （SDS） group and treatment group at 15 in each. SDS model was set up by injection with Reserpine, and the rats were treated with Sijunzi decoction in the treatment group. The level of glycogen in both liver and muscle were detected by histoehemistry methods on 7^th and 14^th respectively. Results showed that the level of glycogen in both SDS group and treatment group was markedly lower than that of control group on 7^th ; the level of glycogen in SDS group was increased obviously and was higher than that of other two groups on 14^th. It is concluded that the changes of glycogen in both liver and muscle could be as the scale indexes during the development of SDS.