应用SSR标记技术鉴定玉米杂交种的纯度

Application of SSR Marker Technique to Purity Testing of Maize

以利用常规盐溶蛋白电泳技术难以进行纯度鉴定的强盛1号玉米杂交种及相应亲本自交系和6对玉米SSR位点引物为材料,筛选出适合该杂交种纯度鉴定的SSR位点引物,结果表明,bilg 116引物的扩增产物呈现双亲互补的特征谱带,易于区分杂交种和亲本自交系。并通过对单子粒DNA提取等技术环节的改进,建立了一套简单、快速、准确、可靠的种子纯度鉴定方法。

In this study, 6 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purity of Qiangsheng No. 1 maize hybrid, which lack a reliable laboratory method for purity testing. The results are as follows： A polymorphic SSR loci, bilg116 has been found that could be applied to test the purity of this hybrid . A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker.