糖基化终末产物对大鼠肾系膜细胞胰岛素样生长因子-1表达的影响

Effects of advanced glycation end products on insulin-like growth factor-1 expression in rat mesangial cells

目的：观察糖基化终末产物（AGEs）对大鼠肾系膜细胞胰岛素样生长因子-1（IGF-1）表达的影响及其与细胞外基质（ECM）成分含量的关系。方法：体外培养正常大鼠肾系膜细胞，分别用不同浓度（0、12．5、25．0、50．0、100．0和200．0mg·L^-1）糖基化牛血清白蛋白（AGEs）处理，相应浓度未经糖化的牛血清白蛋白（BSA）作为对照，ELISA法检测纤维连接蛋白（FN）和Ⅳ型胶原及IGF-1含量。结果：与相应浓度的BSA组比较，12．5～200．0mg·L^-1 AGEs组FN含量明显升高（P〈0．01），100．0～200．0mg·L^-1 AGEs组Ⅳ型胶原含量明显升高（P〈O．01），25．0～100．0mg·L^-1 AGEs组IGF-1蛋白含量明显升高（P〈0．01），200．0mg·L^-1 AGEs组IGF-1含量无明显变化。结论：AGEs引起的细胞外基质积聚可能在一定范围内与IGF-1上调有关。

Objective To investigate the effects of advanced glycation end products （AGEs） on insulin-like growth factor -1 （IGF-1） expression in rat mesangial cells and its relationship with extracellular matrix （ECM） accumulation. Methods Rat mesangial ceils were treated with AGEs-modified bovine serum albumin with different concentrations （0, 12.5 , 25.0 , 50.0 , 100.0 and 200.0 mg·L^-1）, corresponding concentration native bovine serum albumin （BSA） as control. Fibrinectin, collagen IV and ICF-1 protein contents were detected by ELISA. Results Compared with those of corresponding concentration BSA group, FN contents in 12.5-- 200.0 mg ·L^-1 AGEs group increased significantly （P〈0.01）; collagen IV contents in 100.0 200.0 mg·L^-1 AGEs group increased significantly （P〈0.01）~ IGF1 contents in 25.0--100.0 mg·L^-1AGEs group increased significantly （P〈0.01） ; and 200.0 mg ·L^-1 AGEs showed no promotion on IGF-1 expression. Conclusion ECM accumulation induced by AGEs is relevant to IGF-1 in a certain range.