醋酸铅对小鼠骨髓间充质干细胞生长的影响

Effect of lead acetate on growth of murine mesenchymal stem cells

目的:探讨醋酸铅对小鼠骨髓间充质干细胞生长的影响。方法:体外培养法培养小鼠骨髓间充质干细胞,加入40、60和100μmol.L-1醋酸铅,观察其对间充质干细胞成集落(CFU-F)能力的影响及其变化规律。结果:醋酸铅浓度40、60和100μmol.L-1时的成集落率分别为(3.30±0.20)、(2.40±0.10)和(1.57±0.21)/105,与对照组成集落率[(4.20±0.20)/105)]比较差异有显著性(P<0.05);醋酸铅各浓度组间比较,CFU-F集落率差异有显著性(P<0.05),剂量-效应关系分析醋酸铅浓度与CFU-F集落率呈负相关关系(r=-0.960,P<0.01)。体外培养6 d时,对照组集落中细胞多呈现梭形,加铅组细胞多呈不规则形状或圆形。结论:醋酸铅可明显抑制体外培养的骨髓间充质干细胞的成集落率生长并随剂量的增大抑制作用明显增强。

Objective To explore the effect of lead acetate on the growth of murine mesenchymal stem cells in vitro. Methods 40. 00, 60.00 and 100.00μmol·L^-1 of lead acetate were used in the culture of colony-forming unitfibroblast （CFU-F）, the effect on the rate of colony-forming and the rule of variation were observed. Results The rates of colony-forming were （3.30 ± 0.20）, （2.40 ± 0.10） and （1.57 ± 0.21） /10^5, when the doses of lead acetate were 40.00 , 60. 00 and 100.00 μmol·L^-1 and there were significant differences compared with control group （4.20±0.20） /105 , P〈0. 05）. The rates of colony-forming had significant differences between lead acetate with different doses groups （P〈0.05）. Dose-effect relationship was analyzed with linear correlation and correlation coefficient （r=- 0. 960, P〈0. 01）. Colony cells of control group presented fusiform shape while cells of 40. 0 μmol·L^-1 of lead acetate group presented irregularity or round shape cultivated in vitro after 6 d. Conclusion The lead acetate suppresses the rate of colony-forming of CFU-F significantly in vitro and the inhibitory effect increases with the increasing of doses of lead acetate.