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肺炎衣原体的培养及对RAW264.7细胞的感染

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摘要 目的 研究肺炎衣原体(Cpn)的培养及对RAW264.7细胞(人喉癌细胞)的感染。方法 应用低速离心法在Hep-2细胞(小鼠巨噬细胞株)内培养Cpn,光镜及电镜下观察Cpn在Hep-2细胞内增殖情况。提取的Cpn感染RAW264.7细胞,Giemsa染色检测RAW264.7内Cpn感染情况。结果 Cpn能在Hop-2细胞内增殖,提取的Cpn可感染RAW264.7细胞,并在RAW264.7细胞内形成包涵体。结论 在Hep-2细胞内成功培养了Cpn;Cpn能感染RAW264.7细胞。
出处 《卫生职业教育》 2007年第3期111-112,共2页 HEALTH VOCATIONAL EDUCATION
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