罗格列酮增强胰岛素抵抗大鼠胰岛素敏感性作用

Effects of roglitazone on enhancing insulin sensitivity in three rat-models with insulin resistance

目的:研究罗格列酮对多种胰岛素抵抗(insulin resistance,IR)大鼠模型的作用。方法:分别建立2型糖尿病(T2DM)伴IR、高脂血症-IR、地塞米松(DX)诱导IR大鼠3种动物模型,观察罗格列酮对病鼠给药后2h血糖(FBG)、口服葡萄糖耐量试验(OGTT)后2h血糖(2hBG)以及给药后2h空腹血清血糖(FSG)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)、丙二醛(MDA),肿瘤坏死因子-α(TNF-α),胰岛素(Fins)含量及胰岛素敏感指数(ISI),超氧化物歧化酶(SOD)活性等指标的影响。结果:罗格列酮显著降低病鼠FSG,TC,TG,LDL-C和Fins、TNF-α的浓度(P<0.05),显著提高HDL-C含量及ISI(P<0.05),增强胰岛素敏感性。加强SOD活性和降低MDA的含量(P<0.05),增强抗氧化能力。结论:罗格列酮能调整各动物模型所致的糖脂代谢障碍,纠正高脂血症及高胰岛素血症,拮抗病鼠IR,改善其胰岛素敏感性,增强抗氧化作用。

OBJECTIVE To investigate the effects of roglitazone on insulin resistant rats induced by three series of different factors. METHODS Three animal models including type Ⅱ diabetic rats with insulin resistance （T2DR-IR） and dexamethasone-induced insulin resistant rats （DX-IR） and high fat and sugar diet-fed rats with hyperlipemia-insulin resistance were established performed, the effects of roglitazone on serum contents of fasting blood glucose（FBG）, 2-hours blood glucose（2hBG） after oral glucose tolerance test（OGTT）, the serum concentrations of fasting blood glucose （FSG）, low-density lipoprotein cholesterol（LDL-C）, high-density lipoprotein cholesterol （HDL-C）, malondialdehyde （MDA）, total cholesterols （TC）, triacylglycerol （TG）, leptin, tumor necrosis factor-α（TNF-α）, fasting insulin （Fins） and index of insulin sensitivity （ISI）, and activities of superoxide dismutase （SOD） were observed. RESULTS After administration of roglitazone, 2hBG after OGTT was significantly lowered （P〈0. （15） ; the IGT improved; the level of TG, TC, LDL-C were decreased （P〈0. 05） ; the content of HDL-C was heightened （P〈0. 05）; the excessive contents of FSG, TNF-α and Fins were readjust （P〈0. （15）, and enhanced ISI （P〈0. 0） ;the activity of SOD potentiated and content of MDA regressed （P〈0. 05）. CONCLUSION Roglitazone can readjust hyperinsulinemia, correct hyperlipemia and hyperinsulinemia, enhance insulin sensitivity, antagonizing IR, and intensify antioxidation.