中国株HGV部分基因的分子克隆与核苷酸序列分析

Molecular Cloning and Sequencing of 5' Untranslated Region of HGV Genome Isolated From a Chinese Patient

采用ＲＴ－ＰＣＲ对广东地区３３例肝炎病人血清进行ＨＧＶＲＮＡ检测，结果２例（６．１％）阳性。对其中１株输血后ＨＧＶ（ＣＨ－３）基因组５＇端非翻译区（５＇ＵＴＲ）进行分子克隆与测序，并分别与Ｌｉｎｎｅｎ等报道的２株ＨＧＶ（ＰＮＦ２１６１，Ｒ１０２９１）和Ｌｅａｒｙ等报道的ＧＢＶ－Ｃ相比较，其核苷酸序列的同源性分别为８９．２％、８８．７％、８７．１％。本研究首次证实我国华南地区存在ＨＧＶ感染。ＨＧＶ高度保守区５＇ＵＴＲ的分子克隆与核苷酸序列的测定对开展ＨＧＶ感染的基因诊断具有重要意义。

By using RT-PCR,We detected the Serum samples of 33 patients with viral hepatitis in Guangdong China. Two patients were positive for HGV RNA. Partial gene (5' untranslated regioin.5' UTR) of a HGV isolate(Ch-S) from a patient with post-transfusion non A-E hepatitis was cloned,sequenced and compared with some known sequences of HGV PNF2161 strain,R10291 and GBV-C,reported by Linnen,Zhang and Leary,respectively.The nucleotide homology were 89.2% between HGV Ch-S and PNF 2161,88.7% between Ch-S and R10291,87.1% between CH-S and GBV-C.The results of this study confirm the epidemic of HGV infection in Southerm China for the first time. The sequencing of highly conserved 5' UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays.