摘要
应用聚合酶链反应（ＰＣＲ）检测６０例乙型肝炎患者外周血单个核细胞（ＰＢＭＣ）及血清ＨＢＶ·ＤＮＡ，阳性率分别为７６．７％与４５％。尤其在急性乙肝（ＡＨ）或抗ＨＢｅ阳生或抗ＨＢｓ阳性的患者中，ＨＢＶ·ＤＮＡ在ＰＢＭＣ中检出率显著高于血清。提示ＨＢＶ·ＤＮＡ普遍存在于乙肝患者甚ＩＨＢｓＡｇ转阴的病人ＰＢＭＣ中，可能是肝脏病变反复活动及病毒重复感染的来源．因而也导致既往有ＨＢＶ感染史的ＨＢｓＡｇ阴性献血员传播ＨＢＶ的可能。
The presence of HBV·DNA in peripheral blood mononuclear cells(PBMC)and serum samples from 60 patients with different forms of hepatitis B virus (HBV)infection was investigated by PCR, It was found that HBV .DNA could be detected in PBMC in 76.7% and in serum in 45%.In those acute hepatitis B,anti-HBe positive patients or anti-HBs positive patients a positive PCR result was more common in PBMC than in serum, Our results suggest that HBV infection of PBMC is a frequent event during HBV infection and thus suggesting that PBMC may serve as additional reservoirs for reinfection of hepatocytes and may also take a role in the potential transmission of HBV i11 addition to serum when blood donors have remote histories of infection.
