铜绿假单胞菌对亚胺培南耐药机制的研究

Imipenem Resistance Mechanisms in Pseudomonas aeruginosa

目的研究铜绿假单胞菌对亚胺培南耐药机制。方法用PCR法对铜绿假单胞菌亚胺培南耐药相关的金属β-内酰胺酶IMP、VIM基因和外膜蛋白OprD2基因等3种主要耐药基因进行了检测与分析。结果35株耐亚胺培南铜绿假单胞菌IMP和VIM型金属酶基因检测均阴性,35株耐亚胺培南铜绿假单胞菌OprD2基因检测均阴性,但35株铜绿假单胞菌23S rRNA基因扩增阳性。结论研究表明湖北襄樊地区铜绿假单胞菌不产生金属酶,但从遗传学角度证实外膜蛋白OprD2基因缺失是襄樊铜绿假单胞菌对亚胺培南耐药的重要机制。

OBJECTIVE To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa. METHODS PCR method was used to detect and analyze P. aeruginosa imipenem-resistance associated IMP gene and VIM gene of metallo-β-lactamases and outer membrane protein D2 （OprD2） gene. RESULTS All 35 imipenem-resistant P. aeruginosa strains were negative for IMP gene and VIM gene of metallo-β-lactamases; and for OprD2 gene; but 23S rRNA gene of all 35 P. aeruginosa strains was positive. CONCLUSIONS The study suggested that in Xiangfan area, Hubei Province P. aeruginosa doesn＇t produce metallo-β-lactamases, but in genetics it is identified that the loss of outermembrane protein D2（OprD2 ） gene is the essential mechanisms of imipenem resistance in P. aeruginosa in Xiangfan area, Hubei Province.