泌尿生殖道感染支原体培养及药敏分析

Isolation and Antibiotic Susceptibility of Ureaplasma urealyticum and Mycoplasma hominis

目的了解解脲脲支原体(Uu)和人支原体(Mh)在泌尿生殖道感染中的分离及耐药状况,指导临床合理使用抗菌药物。方法应用法国生物梅里埃公司Mycoplasma ISI试剂盒,对730份泌尿生殖道标本进行支原体培养及药敏试验,统计解脲脲支原体和人支原体的检出率,分析其耐药状况。结果男性支原体的检出率为44.7%,女性检出率为46.0%;支原体对环丙沙星(CIP)、氧氟沙星(OFX)、红霉素(ERY)等抗菌药物耐药率较高,其中Uu对上述抗菌药物的耐药率为85.1%,47.1%、24.8%;Mh对上述抗菌药物的耐药率为76.9%、54.2%、54.2%;多西环素(DOX)、普那霉素(PRI)、交沙霉素(JOX)对支原体的敏感度较高,其中Uu对上述抗菌药物的敏感率为95.5%、95.5%、91.3%;Mh为95.8%、95.8%、91.7%。结论治疗支原体感染应选择多西环素、交沙霉素等敏感率高的药物及药敏试验显示敏感的药物,喹诺酮类药物耐药率高,应引起临床重视。

OBJECTIVE To investigate the infectivity and drug resistance of Ureaplasma urealyticum （Uu） and Mycoplasma hominis（Mh）, and instruct reasonable application of antibiotics clinically. METHODS Mycoplasma IST kit was used to detect Uu and Mh and their drug resistance. RESULTS The prevalence of Mycoplasma in males was 44.7 %, in females was 46.0 %. The resistance rate of Uu to ciprofloxacin, ofloxacin and erythromycin was 85.1%, 47.1%, and 24.8 % the resistance rate of Mh to ciprofloxacin, ofloxacin and erythromycin was 76. 9%, 54.2 %, and 54.2 %, respectively. The sensitive rate of Uu to doxycycline, pristinamycin and josamycin was 95.5%, 95.5%, and 91.3% ; the sensitive rate of Mh to doxycycline, pristinamycin and josamycin was 95.8%, 95.8%＇ and 91.7%, respectively. CONCLUSIONS To deal with the infection of Uu and Mh, the antibiotics such as doxycycline, josamycin and others which are sensitive to such strains can be used. Uu and Mh have a high resistance rate to quinolones, which should be paid high clinical attention.