定位显色培养基快速鉴定尿路感染病原菌的临床应用评价

CHROMagar Orientation Medium for Quick Detection of Urinary Tract Pathogens:A Clinical Evaluation

目的探讨显色培养基对尿路感染病原菌的快速鉴定作用。方法对临床疑为尿路感染患者中段尿标本,同时用科玛嘉定位显色培养基和血平板进行对比实验。结果在1 369例临床尿标本中,1 023例标本在两种培养基上都无细菌生长,344例有细菌生长,另外2例在血平板上不生长而在显色培养基上有生长;显色培养基显色鉴定与VITEK-32全自动微生物检测分析仪鉴定结果相符。结论显色培养基对引起尿路感染常见病原菌的生长无抑制作用,两种培养基对病原菌的鉴定结果差异无统计学意义,显色培养基能快速确定病原菌的属别,对及时合理使用抗菌药物和识别混合感染也有一定意义。

OBJECTIVE To investigate the function of CHROMagar orientation medium for quick detection of urinary tract pathogens, METHODS The comparative experiment was performed with CHROMagar orientation medium and 5% sheep blood agar for clinical urine specimens from the patient with suspeetive urinary tract infections, RESULTS Among the total of 1369 clinical urine specimens, 1023 specimens yielded no growth, 344 specimens yielded growth on both media, and 2 other specimens yielded no growth on blood agar but yielded growth on CHROMagar orientation medium, CONCLUSIONS The growth of bacteria is not inhibited by the orientation medium and no significant differences were found in the results between the both media. The orientation medium can quickly confirm the genus of the pathogens, and it has significance for using antibiotics reasonably, One of the greatest advantages of this medium is the easy recognition of mixed cultures,