富硒螺旋藻对大鼠实验性肝纤维化的拮抗效应

Antagonistic effects of Se-rich Spirulina platensis on rat liver fibrosis

目的研究富硒螺旋藻(Se-SP)对大鼠肝纤维化的拮抗作用。方法SD大鼠腹腔注射3%硫代乙酰胺(TAA)溶液复制肝纤维化病理模型,每天灌胃分别补硒(10.8μg)、藻粉(100mg)和富硒藻粉(100mg,其中含硒10.8μg)进行实验干预。实验结束取大鼠血清进行常规肝功能检查,DAN荧光法测定硒(Se)含量,TBA法测定血清过氧化产物丙二醛(MDA)浓度,放射免疫法测定血清透明质酸(HA)水平,比色法检测谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)活性。大鼠肝组织切片HE染色进行病理分析,Masson三色法胶原染色估计肝组织胶原相对含量(RCC%)。结果Se-SP组肝功能指标恢复最好,其血清中Se含量和GPx活性均明显提高(P<0.05),MDA和HA则明显降低(P<0.05)。肝纤维化病理诊断主要为Ⅰ级,肝组织RCC降低了约60%。进一步分析发现血清Se含量与GPx活性呈正相关(r=0.720),与MDA呈负相关(r=0.675)。结论Se-SP对大鼠实验性肝纤维化有较好的拮抗效应,作用机制与增强机体抗氧化水平和增加肝功能贮备有关。

Objective Antagonistic effects of supplement of Se-rich Spirulina platen.sis （Se-SP） on hepatocirrhosis were investigated with the rat model of liver fibrosis induced by intraperitoneal injection 3% thioaeetamide （TAA）. Methods Parameters of routine liver function, content of malondialdehyde （MDA） and activities of glutathione peroxidase （GPx） and superoxide dismutase （SOD） in rat serum were determined by eolorimetry. Content of selenium （Se） was measured by DAN fluorometry method and hyaluronie acid （HA） was detected by radio-immunoassay. Liver fibrosis was diagnosed by HE staining and relative contents of collagen （RCC） were estimated by Masson＇ s trichrome staining. Results Parameters of liver function in Se-SP group were most recovered in all protective groups. Compared with the model groups, contents of MDA and HA were lower, whereas activities of GPx and SOD were higher （ P 〈 0.05） in rats serum of Se-SP group. The RCC in rats liver of Se-SP group were lower than those of the model groups, where the liver fibrosis were identified dominantly to degree Ⅰ according to pathological diagnosis. Moreover, Se content in rats serum had positive correlation （ r = 0. 645） with activity of GPx while a negative correlation （ r = 0. 675） with MDA level. Conclusion The results indicated that Se-SP have detectable antagonistic effects to liver fibrosis, and suggested that enhancement of antioxidation level and liver reserve function might be associated with these effects.