β淀粉样蛋白处理大鼠认知和微量营养素状况的变化

Changes of cognition and micronutrient levels in rats treated by β amyloid protein

目的观察认知功能损伤大鼠体内几种主要维生素和微量元素含量的改变。方法30只雄性SD大鼠随机分为正常对照组、β淀粉样蛋白(Aβ)组和生理盐水组,每组10只。正常对照组不作任何处理,Aβ组双侧海马内注射Aβ(每侧10μg),生理盐水组海马内注射等体积生理盐水。实验周期两周。通过水迷宫试验、避暗试验检测大鼠认知功能;采用原子吸收分光光度法、微量荧光法等方法检测动物血清维生素A、维生素E、维生素C、维生素B2、维生素B6、叶酸及微量元素铜、锌、铁的含量。结果海马内注射Aβ使大鼠认知功能有下降趋势;与正常对照组及生理盐水组比较,Aβ组大鼠水迷宫试验及避暗试验潜伏期均延长,且血清维生素C及铁含量明显下降(P<0.05);各组大鼠其余指标差异均无显著性。结论海马内注射Aβ可导致大鼠认知功能损伤以及血清维生素C、铁含量降低。

Objective To observe the changes of the cognition and micronutrient level in rats treated by β amyloid protein. Methods Thirty male Sprague-Dawley rats were randomly divided into control group, β amyloid group（Aβ） and saline group. The rats were injected Aβ and saline by bilateral intrahippocampus（10μg per lateral）in Aβ group and saline group separately. The experiment lasted 2 weeks. The cognition was identified by water maze and step through test. The contents of serum micronutrients were examined by atomic absorption spectrometry and fluorescence spectrometry. Results Declined cognition and prolonged latency were observed in Aβ group. The contents of serum vitamin C and iron in Aβ group were significantly lower than those of the control group and saline group（ P 〈 0.05） . There was no significant difference among the three groups in other indicators. Conclusion It was seemed that impaired cognition and decreases of serum vitamin C and iron level could be observed in rats treated by β amyloid protein.