上海市女童血清中邻苯二甲酸二丁酯和邻苯二甲酸-2-乙基己酯水平与性早熟关系研究

Study on the di-n-butyl phthalate and di-2-ethylhexyl phthalate level of girl serum related with precocious puberty in Shanghai

目的通过对上海市性早熟女童血清中邻苯二甲酸二丁酯(DBP)和邻苯二甲酸-2-乙基己酯(DEHP)水平变化研究,探讨DBP、DEHP与性早熟女童发病的关系。方法运用气相色谱法测定110例性早熟女童和100例正常儿童血清中DBP和DEHP的含量,同时运用B型超声仪测定性早熟女童与正常儿童的子宫与卵巢体积,然后做性早熟女童血清中DBP和DEHP含量水平与子宫、卵巢体积之间的相关性分析。结果27.3%和22.7%的性早熟女童血清中分别检测出DBP和DEHP,而正常儿童仅4%和3%分别检测出DBP和DEHP。从血清中DBP和DEHP的含量构成比来看,性早熟女童血清中DBP和DEHP的含量构成比均高于正常儿童;性早熟女童子宫、卵巢体积均大于正常儿童子宫、卵巢体积(P<0.05,P<0.05);性早熟女童血清中DBP与子宫呈正相关(r=0.456,P<0.05),与卵巢也呈正相关(r=0.378,P<0.01),性早熟女童血清中DEHP与子宫呈正相关(r=0.382,P<0.05,),与卵巢呈正相关(r=0.689,P<0.01)。结论性早熟女童受DBP和DEHP污染的程度均比正常儿童严重得多,而且DBP和DEHP对子宫、卵巢的体积有影响。

Objective To determine the levels of the di-n-butyl phthalate and di-2-ethylhexyl phthalate in the serum of precocious girls in Shanghai, and study the development relationship between DBP, DEHP and precocious girls, Methods The concentrations of two phthalates （di-n-butyl phthalate, DBP, di-2-ethylhexyl phthalate, DEHP） in serum of 110 precocious girls and 100 normal children were measured by using gas chromatography, in the same time, the volumes of the uteruses and ovaries for precocious girls and normal children were measured by B-ultrasound and then did correlation analysis between the concentrations of DBP, DEHP and the volumes of uteruses and ovaries of precocious girls. Results DBP and DEHP were detected for 27.3% and 22.7% in precocious girls respectively, DBP and DEHP were detected for only 4% and 3% in normal children respectively. From the ratio of DBP and DEHP in serum of precocious girls were higher than normal children. Precocious girls uteruses and ovaries volume were higher than those of normal children respectively（ P 〈 0.05, P 〈 0.05）. DBP in serum of precocious girls had positive correlation with the volumes of the uteruses（ r = 0.456, P 〈 0.05） ,and had positive correlation with volumes of the ovaries（ r = 0.378, P 〈 0.01 ） . DEHP in serum of precocious puberty had positive correlation with the volumes of uteruses（ r = 0.382, P 〈 0.05）, and had positive correlation with volumes of ovaries （ r = 0. 689, P 〈 0.01 ）. Conclusion Precocious girls could be contaminated more severely than normal girls, and DBP, DEHP could influence the volumes of the uteruses and ovaries.