毛细管电泳法和分光光度法测定有机氮源中可溶性无机磷的比较

Comparison of Soluble Inorganic Phosphorus Quantification with Capillary Electrophoresis and Spectrophotometry in Organic Nitrogen Sources

通过优选缓冲液体系,建立了阴离子的毛细管电泳(CE)检测法,以测定天然有机氮源中可溶性无机磷的含量,并与分光光度法进行了比较。结果显示,分光光度法检测限较低,但CE法线性范围更广,结果准确,在自动化快速分析方面亦有较大优势,更适于微生物培养过程中离子含量的动态分析。

A capillary electrophoresis （CE） method was established and optimized to separate anions in organic nitrogen sources. The CE method was applied to determine soluble inorganic phosphorus and the results were compared with those of spectrophotometry. Although spectrophotometry had lower detection limit, the CE method had notable advantages in linearity range, automation and detection speed. The results indicated that the CE method was more fit for the analysis of ions concentration during the fermentation process.