慢性肾衰腹膜透析患者白细胞介素Ⅱ受体的测定

The determinations of Interleukin-2 Receptor in crf patients with peritonealdialysis

本文测定２６例慢性肾衰腹膜透析患者外周血单个核细胞白细胞介素Ⅱ受体（ＰＢＭＣｍＩＬ－２Ｒ）表达、血浆可溶性白细胞介素Ⅱ受体ｓＩＬ－２Ｒ、Ｔ细胞亚群及ＮＫ细胞活性。结果为慢性肾衰腹膜透析患者ｍＩＬ－２Ｒ表达（４１．７±７．６７％）明显低于正常对照组（５２．９８±４．９９％）；ｓＩＬ－２Ｒ水平（７８７．６９±２７９．２３ｕ／ｍｌ）明显高于对照组（３０４．０５±１２４．１２μ／ｍｌ）；Ｔ４／Ｔ８比值和ＮＫ细胞活性也明显低于对照组。相关分析表明ｍＩＬ－２Ｒ表达与Ｔ４呈正相关（ｒ＝０．４５，Ｐ＜０．０５）；而与ｓＩＬ－２Ｒ、ＮＫ活性无关。由于ｍＩＬ－２Ｒ表达减少，ｓＩＬ－２Ｒ水平升高可使ＩＬ－２生物学功能发挥受限，并抑制活化的Ｔ细胞，故推测慢性肾衰腹透患者细胞免疫功能低下可能与此有关。

The exprssion of membrane Interleukin-2 receptor(mIL-2R) on PBMC,plasma soluber Interleukin-2 receptor(sIL-2R),T-1ymphocyte subset and NK activity in 26 CRF patients with peritonealdialysis were measured. The results showed that the patients with peritonealdialysis had markedly decreased expression of mIL-2R (41.7-7.76%) and increased plasma concentrations of sIL-2R (787.69-279.23u/ml) compared with control (52.98-4.99%) and 304.05-124.12u/ml respectively).The ratio of T4/T8, and NK activity in CRF patients with peritoneal dialysis were lower than that of control. Relative analysis between them revealed that mIL-2R had a positive correlation with T4 (r=0.45 P<0.05) and no correlation with sIL-2R and NK activity. The decreased expression of mIL-2R could affect the biological functions of IL-2 and higher level sIL-2R inhibit activated T cell, which might be related with the depression of cell immunity in CRF patients with peritonealdialysis.