青钱柳离体胚的培养及快速繁殖

A Preliminary Study on Embryo Culture and Rapid Propagation in vitro of Cyclocarya paliurus

以青钱柳离体胚为材料,采用正交试验设计对影响其萌发、快速成苗的各种因素进行研究,并进行了诱导增殖试验。结果表明,基本培养基种类是影响青钱柳离体胚萌发和成苗最关键的因素。离体胚萌发的最佳培养基组合为WPM+GA3(10 mg/L)+蔗糖(30 g/L),萌发率可达100%;适宜成苗的培养基组合是WPM+GA3(5 mg/L)+麦芽糖(30 g/L),培养60 d的无菌苗移栽成活率为100%;最适的增殖培养基为WPM+6BA(0.5 mg/L),平均增殖系数为3.45。

The present research was conducted to determine the effects of basal medium, GA3 and sugar on the embryo germination and seedling development of Cyclocarya paliurus by orthogonal design and to induce the obtained shoots to proliferate. The results showed that the basal medium played a determinative role in the embryo germination and seedling development. The best media to promote embryo germination was WPM＋GA3 （10 mg/L）＋sucrose （30 g/L）, just the E6 treatment according to the orthogonal design, on which the percentage of germination was up to 100%, and the optimal media to develop into seedlings was WPM＋GA3（5 mg/L）＋maltose （30 g/L）. Transplanted into the mixture of perlite, peat and vermiculite （1：1：1）, the plantlets were all survived after one month. The optimal induction media for proliferation was WPM＋6- BA（0. 5 mg/L）, and the mean coefficient of proliferation was 3. 45.