UHT杀菌乳中耐热菌的分离与鉴定

Isolation and identification of thermoduric bacteria in UHT sterilized milk

采用2种不同的细菌富集方式对5种不同品牌的UHT(Ultra High Temperature)杀菌牛乳中残留的细菌进行了分离,得到18株细菌,并对它们进行了耐热性和细菌学鉴定试验。结果表明,18株细菌全部耐热,初步将其归为3个属。其中,片球菌属10株,均为戊糖片球菌,肠球菌属3株,1株类鸟肠球菌,2株粪肠球菌;微球菌属5株,1株嗜冷微球菌,4株变异微球菌。虽然这些耐热菌均为非致病菌,但是遇到适宜条件,这些耐热菌会生长繁殖,导致乳产品在贮藏和流通过程中发生变质。

In this investigation （paper）,the residual bacteria in five different brands UHT sterilized milk were isolated by two different bacteria enrichment means. As a result, 18 strains bacteria were gained, then the thermostability and identification experiments of them were operated. The results indicated that all bacteria strains can resisted the heat operation and they belonged to 3 genus：Pediococcus,10 strains were P.pentosaceus; Enterococcus,lstrain was E.pseudoavium and 2 strains were E.faecalis; Micrococcus,1 strain was M.cryophilus and 4 strains were M. varians. These thermoduric bacteria were nonpathogenic bacteria, but they could grow and multiply as well as lead to milk deterioration in storage and circulation in eligible condition.