与烟草细胞质雄性不育相关的线粒体基因atp9的mRNA研究

Studies on atp9 mRNA related to cytoplasmic male sterility in tobacco

已知导入未编辑atp9 mRNA的烟草表现细胞质雄性不育(CMS),因此认为线粒体基因atp9是引起高等植物CMS的主要基因。为了解atp9在CMS中的作用机制,从3对烟草不育系及其同型保持系中提取atp9,利用实验与理论结合来分析其mRNA在编辑前后以及在不育系及其同型保持系中的一维、三维信息差别。结果表明,atp9mRNA一维信息方面的差异,更重要的是二级结构的差异和稳定性,可能是影响ATP合成而导致CMS的根本原因。

It was reported that the transgenic tobacco plants transformed with an unedited copy of the mitochondrial atp9 gene exhibited the trait of the cytoplasmic male sterility （CMS） phenotype. Therefore, it was believed that atp9 gene functioned as a dominant gene on CMS in higher plants. To understand the CMS mechanism caused by unedited form of atp9 deeper,atp9 gene was cloned from three sterile tobacco lines and their isogenic fertile lines. The differences in one-or three-dimensional information between unedited states and edited ones, or between sterility and fertili- ty were analyzed. The results suggested that the differences in one-dimensional sequences, especially in secondary structures and their stabilities might possibly be the underlying reason resulting in CMS because of affecting ATP synthesis.