巴戟天组织培养和快速繁殖研究

Tissue culture and rapid proliferation of Morinda of ficinalis How

以巴戟天顶芽及嫩茎节段为外植体，以MS为基本培养基，通过不同的激素种类和浓度配比，建立巴戟天组培快繁体系。结果表明，外植体表面消毒以70％酒精预处理60s，再用0．1％HgCl2浸泡10min，效果较好，茎节为外植体优于顶芽。培养基MS＋BA1．0mg／L＋IBA0．05mg／L利于诱导出芽，可用于初代培养；MS＋BA1．0mg／L＋IBA0．2mg／L利于形成丛生芽，用于继代增殖，繁殖系数6．0／50d；1／2MS＋IBA0.4～0.8mg／L适宜诱导生根获得再生植株，生根率100％；生根苗移栽于排水良好的火土或砂土中，成活率90％。

The tissue culture and rapid proliferation techniques of Morinda officinalis How were studied by using apical buds and tender stems as explants. The explants were cultivated in different MS medium with different types and concentrations of plant hormones. The results showed that the most suitable procedure for sterilization was to soak explants into 0. 1% HgCl2 solution for 10 minutes after pretreatment with 70% alcohol for 60 seconds. The tender stems were superior to apical buds in vitro culture procedure. The medium MS ＋IBA 0.05 mg/L＋BA 1.0 mg/L was effective to induce shooting and suitable for the first generation cultivation; MS＋IBA 0.2 mg/L＋BA 1.0 mg/L could effectively induce fasciculate buds and be suitable for subculture and proliferation, the propagation coefficient was 6.0 every 50 days; the most optimum rooting medium was 1/2MS＋IBA 0.4～0.8 mg/L, and the rooting rate was 100%. The rooting seedlings were transplanted in sand or burnt soil with plant ash. The highest survival rate was 90%.