下颌前伸对大鼠髁突软骨VEGF表达影响的研究

The expression of vascular endothelial growth factor in the condyle during forward mandibular positioning

目的:探讨引导大鼠下颌前伸后,髁突软骨中血管内皮细胞生长因子(VEGF)表达水平的变化。方法:50只35d龄雌性SD大鼠随机分为5组,每组随机分为实验组(5只)和对照组(5只);实验组大鼠24h佩戴自制功能性矫治器引导下颌前伸;实验后3d、7d、14d、21d和30d分别处死各组大鼠;免疫组化方法检测髁突软骨中VEGF的表达。结果:对照组髁突软骨VEGF表达随时间延长而减弱,后部区域VEGF表达强于前部和中部;实验后第14d,21d和30d实验组VEGF表达强于相应对照组(P<0.05),其中14d时软骨后部VEGF表达较对照组增强幅度最大。结论:下颌前伸后大鼠髁突软骨细胞VEGF表达增强,说明其参与了软骨内骨化过程。

Objective： To investigate the temporal pattern of expression of Vascular Endothelial Growth Factor （VEGF） in the condyle of rat during forward mandibular positioning. Method： Fifty 35-day-old female Spague-Dawley （SD） rats were randomly divided into 5 groups.Each group consisted of five rats with bite-jumping appliance and five untreated.One group was sacrificed on days 3, 7, 14, 21 and 30 respectively.VEGF immunostaining was performed to detect the expression level of VEGF. Result： The expression level of VEGF in control groups decreased during the experimental periods with higher expression in posterior region compared to anterior and middle regions.Mandibular advancement in rats caused a gradual increase in the expression of VEGF when compared to the control group （P〈0.05）, with the highest amount of changes in the posterior region on Day 14. Conclusion： Mandibular advancement in rats caused cellular changes of condyle, which led to increased VEGF expression and endochondral ossification.