摘要
目的 探讨转化生长因子-β(TGF-β)(-509、+869、+915)位点基因多态性与尘肺易感性的关系。方法 采用1:1配对的病例一对照研究方法。病例组为117名Ⅰ期尘肺患者,对照为与病例年龄相差不超过5岁、同性别、同民族、同一工作地点、开始接尘时间和累积接尘工龄相差不超过2年的非尘肺接尘工人117名。应用限制性片段长度多态性分析(PCR-RFLP)检测TGF-β3个位点基因型。结果病例组TGF-β(-509)位点CC、CT和啊基因型频率分别为22.2%、43.6%和34.2%,与对照组比较,差异有统计学意义(OR=1.390,P〈0.05);TGF-β(+869)位点基因型频率与对照组比较,差异无统计学意义(OR=1.203,P〉0.05)。病例组TGF-13(+915)位点GG、GC基因型频率分别为70.9%、29.1%,与对照组比较,差异有统计学意义(OR=1.455,P〈0.05)。病例组-509*C等位基因频率低于对照组,+915*C等位基因频率高于对照组,差异均有统计学意义(P〈0.05);同时携带-509的CC基因型和+915的GG基因型在病例组分布频率为12.8%,对照组为29.9%,差异有统计学意义(P〈0.01)。结论 TGF-β(-509)CC为保护性基因型,TGF-β(+915)GC基因型是尘肺的易感基因型。同时携带TGF-β(-509)*T和(+915)*C等位基因的接尘人群更易患尘肺。
Objective To explore the association between genetic polymorphisms of transforming growth factor-β(TGF-β) and susceptibility to pneumoconiosis. Methods One hundred and seventeen patients with pneumoconiosis were selected as case.The control group was workers exposed to dust but without pneumoconiosis who had the same sex,nationality,and workshop or work site as the case.The differences in the age and cumulative exposure time between the case and control group were not more than five years and two years, respectively. The case matched with the control according to 1:1. Polymerase chains reaction-restriction fragment length polymorphism was used to determine the frequencies of TGF-β genes in the two groups. Results The frequencies of this TGF-β(-509) genotypes were CC(22.2%), CT (43.6%) and TT (34.2%) in cases, which was significantly different from the control group, respectively (OR=1.390, P〈0.05). There was no significant difference in the frequency of TGF-β +869 genotypes and allelic between case and control (P〉0.05).The frequencies of the TGF-β(+915) genotypes in case [GG(70.9%), GC(29.1%)] were significantly different from the control group(OR=1.455 ,P〈0.05).The frequency of TGF-13(+915) * C allele in the case and the control was 14.5% and 8.5%, respectively (P〈0.05).The frequencies of carrying TGF-β(-509)CC and (+915)GG genotypes were 12.8% and 29.9% in the case and the control. The frequencies of carrying TGF-13(-509) * T and (+915) * C alleles were 9.8% and 5.1% in case and control (P〈0.05). Conclusion TGF-13(-509)CC genotype may be the protective factor for the pneumoconiosis. TGF-13(+915)GC genotype may be a susceptible factor for the pneumoconiosis. The workers of carrying TGF-13(-509)* T and (+915) * C alleles are more susceptible to pneumoconiosis.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2007年第1期1-4,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家安全生产监督管理总局资助项目(2002)
关键词
转化生长因子-Β
多态性
单核苷酸
尘肺
Transforming growth factor beta
Polymorphism, Single nucleotide
Pneumoconiosis