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低氧诱导胎肝间质细胞表达胰岛素样生长因子 被引量：1

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摘要 目的观察低氧培养对胎肝间质细胞表达胰岛素样生长因子1(IGF-1)的情况。方法取第5代生长至次融合状态的小鼠胎肝间质细胞用含95%N2和5%CO2混合气培养,半定量RT-PCR和ELISA检测IGF-1mRNA转录与IGF-1浓度。结果低氧培养2hIGF-1mRNA即显著增高,4h后IGF-1与β-actin两者RT-PCR产物信号比从常氧培养的(7.59±2.63)%增加到(34.12±8.17)%,IGF-1蛋白也显著增高;低氧培养8hIGF-1蛋白从常氧培养的(17.69±3.81)ng/mg总蛋白增加到(94.53±15.86)ng/mg总蛋白(P<0.01)。结论低氧可诱导胎肝间质细胞表达IGF-1,提示低氧在组织缺血损伤疾病中的潜在治疗价值。
作者 刘革修 张洹 何冬梅 谭广销
机构地区 暨南大学医学院血液病研究所
出处 《中国老年学杂志》 CAS CSCD 北大核心 2007年第2期129-131,共3页 Chinese Journal of Gerontology
基金 暨南大学引进优秀人才项目基金资助(NO.51204001)
关键词 低氧 胰岛素样生长因子 小鼠 基因表达 间质细胞
分类号 R743.31 [医药卫生—神经病学与精神病学]
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参考文献9

  • 1Chen J,Zhang ZG,Li Y,et al.Intravenous administration of human bone marrow stromal cells induces angiogenesis in the ischemic boundary zone after stroke in rats[J].Circ Res,2003 ;92(6):692-9.
  • 2Lu D,Sanberg PR,Mahmood A,et al.Intravenous administration of human umbilical cord blood reduces neurological deficit in the rat after traumatic brain injury[J].Cell Transplant,2002; 11 (3):275-81.
  • 3Chen X,Katakowski M,Li Y,et al.Human bone marrow stromal cell cultures conditioned by traumatic brain tissue extracts:growth factor production[J].J Neurosci Res,2002;69(5):687-91.
  • 4刘平平,张洹,刘革修.体外诱导小鼠胎肝间充质细胞向神经元样细胞分化的研究[J].第二军医大学学报,2003,24(9):950-953. 被引量：4
  • 5余卫,张洹,何冬梅.体外诱导小鼠胎肝间充质干细胞向胰岛B样细胞分化的研究[J].第二军医大学学报,2004,25(8):827-830. 被引量：12
  • 6Guan J,Bennet L,Gluckman PD,et al.Insulin-like growth factor-1 and post-ischemic brain injury[J].Prog Neurobiol,2003; 70(6):443-62.
  • 7Anderson MF,Aberg MA,Nilsson M,et al.Insulin-like growth factor-Ⅰ and neurogenesis in the adult mammalian brain[J].Brain Res Dev Brain Res,2002; 134(1-2):115-22.
  • 8Kazanis I,Bozas E,Philippidis H,et al.Neuroprotective effects of insulinlike growth factor-Ⅰ (IGF-Ⅰ) following a penetrating brain injury in rats[J].Brain Res,2003 ;991 (1-2):34-45.
  • 9Lopez-Lopez C,LeRoith D,Torres-Aleman I.Insulin-like growth factor Ⅰ is required for vessel remodeling in the adult brain[J].Proc Natl Acad Sci USA,2004; 101 (26):9833-8.

二级参考文献25

  • 1Massimo S,Paolo O,Elena B,et al. Long-term cultures of human fetal liver cells..a three-dimensional experimental model for monitoring liver tissue development[J]. J Hepatol, 1998,28(3)..480-490.
  • 2Kabos P,Ehtesham M,Kabosova A,et al. Generation of neuralprogenitor cells from whole adult bone marrow[J]. Exp Neurol,2002,178(2):288-293.
  • 3Zigova T, Song S, Willing AE, et al. Human umbilical cord blood cells express neural antigens after transplantation intothe developing rat brain [J]. Cell Transplant, 2002,11 (3) :265-274.
  • 4Choi JU,Yoon DH,Yeon DS,et al. Neural phenotype expression of cultured human cord blood cells in vitro[J]. Neuroreport, 2001,12(16):3523-3527.
  • 5Bain G, Kitchens D, Yao M, et al. Embryonic stem cells express neuronal properties in vitro[J]. Dev Biol, 1995,168(2) :342-357.
  • 6Chagraoui J,Lepage NA,Anjo A,et al. Fetal liver stroma consists of cells in epithelial-to-mesenchymal transition [J].Blood,2003,101 (8) :2973-2982.
  • 7James ED,Pierre C. Origin and differentiation of human and murine stroma[J]. Stem Cells,2002,20(3) :205-214.
  • 8Tocci A,Rezzoug F,Wahbi K,et al. Fetal liver generates low CD4 hematopoietic cells in murine stromal cultures[J]. Blood,1995; 85(6):1463-1471.
  • 9lshii K, Katayama M, Hori K, et al. Effects of 2-mercaptoethanol on survival and differentiation of fetal mouse brain neurons cultured in vitro[J]. Neurosci Lett, 1993,163 (2) : 159-162.
  • 10Allenby G, Bocquel MT, Saunders M, et al. Retinoic acid receptors and retinoid X receptors:interactions with endogenous retinoic acids [J]. Proc Natl Acad Sci, 1993,90( 1 ):30-34.

共引文献13

同被引文献22

  • 1刘革修,张洹,何冬梅,谭广销.低氧诱导胎肝间质细胞表达碱性成纤维生长因子[J].第二军医大学学报,2005,26(9):1012-1014. 被引量：3
  • 2刘革修,张洹,何冬梅,谭广销.低氧诱导胎肝基质细胞表达转化生长因子β1表达升高[J].第二军医大学学报,2005,26(10):1148-1151. 被引量：1
  • 3刘革修,张洹,何冬梅.人骨髓间充质细胞在低氧培养条件下脑源性神经营养因子的表达[J].中国临床康复,2006,10(29):1-3. 被引量：2
  • 4Noort WA, Feye D, Van Den Akker F, et al. Mesenchymal stromal cells to treat cardiovascular disease:strategies to improve survival and therapeutic results [ J ]. Panminerva Med,2010,52(1) :27 -40.
  • 5Kode JA, Mukherjee S, Joglekar MV, et al. Mesenchymal stem cells:immunobiology and role in immunomodulation and tissue regeneration [ J ]. Cytotherapy, 2009,11 ( 4 ) : 377 - 39l.
  • 6Naveiras O, Nardi V, Wenzel PL, et al. Bone - marrow adipocytes as negative regulators of the haematopoietic microenvironment [ J ]. Nature, 2009, 460 ( 7252 ) : 259-263.
  • 7Ksiazek K. A comprehensive review on mesenehymal stem cell growth and senescence [ J ]. Rejuvenation Res, 2009, 12(2) :105 - 116.
  • 8Fehrer C, Lepperdinger G. Mesenchymal stem cell aging [ J ]. Exp Gerontol,2005,40 ( 12):926 - 930.
  • 9Krishnamurthy J, Torrice C, Ramsey MR, et al. Ink4a/ Arf expression is a biomarker of aging[ J]. J Clin Invest, 2004, 114(9) :1299 - 1307.
  • 10Ohtani N, Yamakoshi K, Takahashi A, et al. The p16^INK4a - RB pathway: molecular link between cellular se- nescence and tumor suppression [J]. J Med Invest,2004, 51 (3 -4) :146 - 153.

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中国老年学杂志
2007年 第2期

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