转基因抗虫棉苗期氮代谢

Nitrogen metabolism of transgenic Bt cotton and transgenic Bt-CpTI cotton at seedling stage.

以3种不同转基因抗虫棉及其亲本对照为材料,研究了盆栽条件下不同品种棉花苗期氮代谢特征。结果表明与非转基因棉花比较,转Bt基因棉Z30叶片内的硝酸还原酶活性、肽酶活性、游离氨基酸含量均没有明显变化,但转氨酶活性显著增加(增幅为14.03%),可溶性蛋白含量显著减少(降幅为26.29%)。双价转基因棉花CCRI41叶片内除可溶性蛋白含量没有明显变化,其它所测指标均发生了显著的改变。双价转基因棉花SGK321叶片内硝酸还原酶活性、转氨酶活性、氨基酸含量没有明显变化,但肽酶活性和可溶性蛋白含量均显著增加(增幅分别为7.01%和121.32%)。随着转入基因的多样化,其可能引发转基因棉花产生的非预期效应更加不确定与复杂。

Nitrogen metabolism of Bt transgenic cotton and Bt-CpTI transgenic cotton at seedling stage was studied by pot experiment. The results showed that the characters of nitrogen metabolism in transgenic cotton leaves changed and differed in different transgenic events. In comparing with non-transgenic treatment, there were not significant differences in the activities of nitrate reductase and peptidase and in the content of free amino acid, but there was a significant increase in GTP activity and a significant decrease in free amino acid in transgenic Bt cotton leaves. Except for the soluble protein content, all the other characteristics of nitrogen metabolism of Bt-CpTI transgenic cotton ZA1 changed significantly. Both the peptidase activity and the soluble protein content in Bt-CpTI transgenic cotton SK321 increased significantly, but the activities of nitrate reductase and GTP and the content of free amino acid in that treatment didn＇ t change obviously. The unantichipated effects of transgenic crops would be more uncertain and complex along with diversification of transgenic genes.