丙烷脒对番茄灰霉病菌超微结构的影响

Effects of propamidine on the ultrastructure of Botrytis cinerea Pers

利用电子显微技术研究了丙烷脒(propamidine)对番茄灰霉病菌(Botrytiscinerea Pers.)菌丝形态和细胞结构的影响.研究发现丙烷脒不仅影响菌丝的生长,而且引起菌丝形态和细胞结构一系列的变化.丙烷脒处理番茄灰霉病菌菌丝后,引起病菌菌丝形态和细胞结构的变化主要包括菌丝分枝增多,并呈不规则的膨大和缢缩,细胞壁出现不规则的加厚,顶端加厚尤其严重,线粒体数目急剧增多且出现不规则的膨大现象,随着处理时间的延长,细胞出现空泡化和电子致密度增加现象.这些细胞学变化特征最终导致菌丝细胞解体死亡.

Effects of propamidine on cell ultrastructure of Botrytis cinerea Pers. were examined by means of electron microscopy techniques. The studies revealed that propamidine not only inhibited the hyphal growth, but also caused a series of marked hyphae morphological and cell structural alterations. These changes are generally irregular swelling, excessive branching and many tumors on the surface of hyphale, considerable thickening of the hyphal cell walls, particularly at the hyphal tip region, vacuoles and electrondense bodies increasing, excessive mitochondrion irregular swelling, and so on. Above cytological change resulted in the necrosis of hyphal cell.