含ipt基因根癌农杆菌转化大豆萌动种胚的同工酶分析

STUDIES ON TRANSFORMATION OF SEED-GERMINATING EMBRYOES OF SOYBEAN BY A.TUMEFACIENS MEDIATED IPT GENE ⅡAnalysis of the Isozymes of the Seedling Stage Roots

本实验以大豆萌动种胚为受体，用含有ＣａＭＶ３５ｓ启动子－Ｇｕｓ基因－ｉｐｔ基因－Ｎｏｓ基因的融合基因根癌农杆菌ＬＢＡ４４０４进行转化，通过对转化及对照植株苗期根的过氧化物酶同工酶及酯酶同工酶分析，结果表明酶谱有明显差异，酯酶同工酶有一条Ｒｆ为０．５４０的差异带，过氧化物酶同工酶有二条Ｒｆ０．５１４，Ｒｆ０．５３２的差异带；综合分析，上述三条差异带都显示的转化植株占所分析转化植株的１４．４％。用此法可作为转基因植株早期筛选方法之一。

The transformation of the seed--germinating embryoes of soyhan was conduce with theAtumefactcens stlain LBA4404 cotaing CaMV 35s promoter -- Gus -- ipt -- Nos fosion gene.The analysis of the peroxidase isozymes and esterase isozymes of the seedling stage rootsof both the usnsforrned and control plants indicatalf The isozymes pattern between thethensformed and control plants presented distinct difference: The porokidase isozymes showedtwo different bands, Rf 0.514 and Rf 0.532, The esterase isozymes showed one different band:Rf 0.540. To sum up, the transformed plants shing both isozymes different bands account for14.4% of the total ones which were analysed. This can be used as one of the methods for earlyscreening of transgenic plants.