摘要
以发根农杆菌30148为材料,设计了rolC基因的特异性引物,应用PCR技术,扩增出了正确序列的rolC基因585 bp片段,并利用pUCm-T载体对此片段进行了克隆,并进行了序列分析。所克隆rolC基因与已发表的序列相比较,核苷酸序列的同源性达到100%,但另一个克隆中部分碱基发生了改变,同源性达到99%。
Ri plasmid was developed and a 585bp fragment of rolC gene was amplified by PCR via primer of rolC gene, and the fragment was cloned into vector pUCm-T. Comparison with previously published nucleotide sequence showed that the homology was 100%. But another clone gene nucleotide sequence showed that the homology was 99% with it.
出处
《西北林学院学报》
CSCD
北大核心
2007年第2期72-75,共4页
Journal of Northwest Forestry University
基金
河北省自然科学基金(302312)
