摘要
通过对杠柳叶的化学成分系统预试,检测出杠柳叶中存在酚性成分、有机酸、皂苷、黄酮类及其苷类、植物甾醇、三萜类化合物。同时以生物活性跟踪法测定了杠柳叶萃取物对三龄粘虫和菊小长管蚜的杀虫活性。结果表明,乙酸乙酯萃取物的活性最高。乙酸乙酯萃取物经硅胶柱层析分离后F3组分的触杀活性最高,72 h对粘虫触杀LC50是2.903 2 mg.mL-1,24 h对蚜虫触杀的LC50为0.526 3 mg.mL-1。
Through preliminary systematic test on chemical composition of Periploca sepium leaves, it was found that the leaves containsed phenols, organic acids, saponins, flavonoids and its glycosides, steroids, and triterpenoids. Meanwhile, insecticidal activities of the extracts from P. sepiurn leaves against Mythimn separate and Macrosiphoniella sanborni were measured by bioactivity-guided sepearation. The results showed that ethyl acetate fraction was more active than others. After ethyl acetate fraction was epearted in the silica gel column, fraction No. 3 exhibited the strongest contact toxicity with LC50 2. 903 2 mg · mL^-1 in 72 hours against M. separate, and 0. 526 3 mg · mL^-1 in 24 hours against M. sanborni.
出处
《西北林学院学报》
CSCD
北大核心
2007年第2期142-145,共4页
Journal of Northwest Forestry University
基金
陕西省自然科学基金(2003C125)
国家林业局黄土高原林木培育重点实验室基金(K03-01)
关键词
杠柳
系统预试
提取物
杀虫活性
Periploca sepium
preliminary systematic test
extraction
insecticidal activity