补阳还五汤质量标准测定及对中风后遗症“气虚血瘀”大鼠病证模型血常规的影响

The Quality Standard of Bu Yang Huan Wu Decoction and its Effects on Blood Routine Examination of Ischemic Stroke Sequela Rats Which Lack of Qi and with Blood Silted

目的：研究补阳还五汤水提物中黄芪甲苷、阿魏酸舍量的测定，作为该方的质量控制标准；复制中风后遗症“气虚血瘀”大鼠模型，观察补阳还五汤对其血常规的影响，从而探讨补阳还五汤在治疗中风后遗症方面的作用机制。方法：补阳还五汤采用传统煎法，黄芪甲苷采用高效液相色谱-蒸发光散射检测。阿魏酸采用高效液相色谱法。用仪器分析法检测血常规。结果：黄茂甲苷在0．24～6．00ug与峰面积积分值呈良好线性关系（r=0．9999），平均回收率100．79％。阿魏酸在0．08—0．24ug与峰面积积分值呈良好线性关系（r=0．9999），平均回收率101．76％。对血常规有影响，可降低白细胞，升高红细胞和血红蛋白，降低过多血小板数目。结论：补阳还五汤可提高免疫机能，改善气虚血瘀和能量代谢，改善循环功能，黄芪甲苷、阿魏酸的含量，可作为该方的质量控制标准。

Objective： Study the method of test astragaloside and ferulic acid in Bu Yang Huan Wu Decoction,to set the Quality Standard ofBu Yang Huan Wu decoction;Make the stroke sequela models of rats which are lack of Qi and with blood silted, study the effects on blood routine examination by Bu Yang Huan Wu decoction. Methods ： HPLC was used to assay the contents of astragaloside and ferulic acid, instrumental method was used for blood routine examination. Results ： the contents of astragaloside is between 0.24 - 6.00ug , the contents of ferulie acid is between 0.08 - 0.24ug; the results showed that Bu Yang Huan Wu decoction reduce white blood cell; ascend red blood cell and hemoglobin ; reduce platelet. Conclusion： Bu Yang Huan Wu decoction is helpful to reduce blood viscosity and improve microcirculation, enhance immunological,the contents of astragaloside and ferulic acid may take as quality standard ofBu Yang Huan Wu decoction.