不同中医辨证分型胃癌细胞拓扑异构酶Ⅱ表达的实验研究

The Empirical Study of the DNAtopoll Expression of Differentiation of Symptoms and Signs for Classification of Syndrome of Gastric Carcinoma

目的:检测不同证候类型胃癌细胞拓扑异构酶Ⅱ(topoⅡ)的表达。方法:原发性胃癌标本52例。中医辨证分为肝胃郁热型、脾胃虚寒型、瘀毒内阻型、痰湿凝结型,SP免疫组织化学染色检测拓扑异构酶Ⅱ的表达。结果:topoⅡ标记指数与胃癌中医辨证各分型均有明显相关性,其中以瘀毒内阻型topoⅡ表达最强。结论:说明瘀毒内阻型胃癌细胞对于DNA调节控制及细胞核内核酸代谢过程功能较强,同对各种肿瘤抗癌药物敏感性表现也较高。

Objective： To exam the DNAtopo Ⅱ expression of different syndrome of gastric carcinoma. Methods ： There are 52 examples of syndrome of traditional Chinese medicine include stagnated heat type of liver and stomach, insufficiency of spleen-YANG, deficiency cold of the stomach,the internal resistance of poison, and the condense of phlegmatic hygrosis, sp immunohistochemistry stain detect the DNAtopo Ⅱ expression. Results ： There are obviously dependability in the label indexes of topo Ⅱ and every traditional Chinese medicine groupings of gastric carcinoma, mostly expression in topo Ⅱ of the internal resistance of poison. Conclusion ： To DNA controUability and the process of cell intranuclear liver nucleic acid metabolism,the gastric carcinoma ceils of the internal resistance of poison have more powerful ability, arid more sensitive to anticancer drugs of various kinds tumors.