摘要
本文对免疫酶组织化学的样品制备程序和染色方法做了详细的阐述。用直接法、间接法和ABC法,对人小肠免疫酶的定位,进行了光镜和电镜的观察,染色阳性反应显著,获得了满意的效果。并对染色技巧做了分析和探讨。
In this artical, the procedures of specimen preparation and the staining method for immunoenzyatic
histochemistry has been elaborated. With direct, indirect and ABC methods, the local distribution of immunoenzyme in the mueosa of small intestinal in human has been observed in LM and EM, the positive reac-tion of staining is remarkable with satisfied results. Also the skill of staining has been analyzed and investigated.
出处
《电子显微学报》
CAS
CSCD
1990年第4期14-19,共6页
Journal of Chinese Electron Microscopy Society
关键词
小肠粘膜
免疫酶
光镜
定位
电镜
Horseradish Peroxidase(HRP)
Diaminobenzidine(DAB)
Peroxidase-anti-peroxidase(PAP)
Avidin-Peroxidase-Biotin Complex(ABC)