热休克蛋白70-HBcAg（18～27）蛋白复合物对乙型肝炎病毒转基因小鼠的免疫研究

Immune response to HSP70-HBcAg（18～27） complex in HBV transgenic mice

目的研究结核杆菌热休克蛋白（HSP）70-HBcAg（18～27）蛋白复合物对HBV转基因小鼠免疫应答的影响。方法体外构建HSP70-HBcAg（18-27）蛋白复合物，以HBV转基因小鼠为实验对象，进行体内免疫学功能研究。结果HSP70-HBcAg（18-27）蛋白复合物使HBV转基因小鼠外周血及脾中CD4^＋ T淋巴细胞和CD8^＋T淋巴细胞大量增殖，诱导较强的抗原特异性CTL应答，并活化自然杀伤细胞、树突状细胞参与应答反应。结论HSP70-HBcAg（18-27）蛋白复合物具有免疫原性，能够增强转基因鼠细胞免疫应答能力，有望作为蛋白疫苗用于慢性乙型肝炎的免疫治疗。

Objective To study the cellular immune response to 70-HBcAg（18～27） complex in HBV transgenic mice. Method 70-HBcAg（18～27） complex was reconstituted in vitro, then it was injected into HBV transgenic mice to observe the cellular immune response. At the same lime, we investigated whether 70-HBcAg（18～27） complex could generate antigen specific cytotoxic T lymphocyte responses in spleen cells. Results Our results demonstrated that 70-HBcAg（18～27） complex increased levels of CD4^＋ and CD8^＋ T cells in the spleens and peripheral blood ofHBV transgenic mice, and the complex also activated dendritic and natural killer cells. Conclusion HSP70-HBcAg（18～27） complex has an immunological antigenicity in raising the immunorespense to chronic HBV infection in HBV transgenic mice. HSP70-HBcAg（18～27） complex might be considered as a candidate for further studies on its role as a therapeutic vaccine against chronic HBV infection in humans.