期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

子宫内膜异位症组织中MMP-2和MMP-9基因表达的意义 被引量：2

Expression of MMP-2 and MMP-9 in endometriosis
下载PDF
导出
摘要 目的:探讨MMP-2和MMP-9在子宫内膜异位症发生中的作用。方法:采用免疫组织化学SP法研究25例子宫内膜异位症和22例正常子宫内膜组织中MMP-2及MMP-9的蛋白表达情况;采用RT-PCR法研究33例子宫内膜异位症的异位子宫内膜组织和30例正常子宫内膜组织中MMP-2和MMP-9基因的mRNA表达情况。结果:子宫内膜异位症组中的MMP-2和MMP-9的蛋白表达及mRNA表达均明显高于各自的正常子宫内膜对照组(P<0.05)。结论:MMP-2和MMP-9在子宫内膜异位症的发病过程中可能起重要的作用。 AIM ： To evaluate the role of matrix metalloproteinase 2 and 9 （ MMP - 2 and MMP - 9 ） in the pathogenesis of endometriosis. METHODS： The expression of MMP-2 and MMP-9 protein in 25 specimens of endometriosis and 22 specimens of normal endometrium were studied by immunohistochemical SP, while the expression of MMP - 2 and MMP -9 mRNA in 33 specimens of endometriosis and 30 specimens of normal endometrium were further assessed by reverse transcription polymerase chain reaction （RT -PCR）. RESULTS ： The protein and mRNA expression levels of MMP- 2 and MMP - 9 in endometriosis were significantly higher than that in normal endometrium, respectively （ P 〈 0. 05 ）. CONCLUSION： MMP- 2 and MMP-9 maybe play an important role in the pathogenesis of endometriosis.
作者 孔丽娜 孙青 李书勤 罗永红 李铁臣 卢林明
机构地区 皖南医学院附属弋矶山医院妇产科 皖南医学院遗传医学研究室 皖南医学院附属弋矶山医院病理科
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2007年第2期379-383,共5页 Chinese Journal of Pathophysiology
基金 安徽省教育厅自然科学研究项目资助(No.2003kj313)
关键词 子宫内膜异位症 免疫组织化学 明胶酶A 明胶酶B Endometriosis Immunohistochemistry Gelatinase A Gelatinase B
分类号 R711.74 [医药卫生—妇产科学]
  • 相关文献

参考文献14

  • 1Efstathiou JA,Sampson DA,Levine Z,et al.Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model[J].Fertil Steril,2005,83(1):171 -181.
  • 2孙青,孔丽娜.子宫内膜异位症与肿瘤转移基因关系的研究进展[J].国外医学（遗传学分册）,2004,27(3):184-187. 被引量：16
  • 3Hudelist G,Lass H,Keckstein J,et al.Interleukin 1 alpha and tissue -lytic matrix metalloproteinase-1 are elevated in ectopic endometrium of patients with endometriosis[J].Hum Reprod,2005,20(6):1695-1701.
  • 4Lockhat FB,Emembolu JE,Konje JC.Serum and peritoneal fluid levels of levonorgestrel in women with endometriosis who were treated with an intrauterine contraceptive device containing levonorgestrel[J].Fertil Steril,2005,83(2):398 -404.
  • 5Ueda M,Yamashita Y,Takehara M,et al.Gene expression of adhesion molecules and matrix metalloproteinases in endometriosis[J].Gynecol Endocrinol,2002,16 (5):391-402.
  • 6Huang HF,Hong LH,Tan Y,et al.Matrix metalloproteinase 2 is associated with changes in steroid hormones in the sera and peritoneal fluid of patients with endometriosis[J].Fertil Steril,2004,81(5):1235 -1239.
  • 7Mulayim N,Savlu A,Guzeloglu-Kayisli O,et al.Regulation of endometrial stromal cell matrix metalloproteinase activity and invasiveness by interleukin-8[J].Fertil Steril,2004,81 (Suppl 1):904 -911.
  • 8Cox KE,Piva M,Sharpe-Timms KL.Differential regulation of matrix metalloproteinase-3 gene expression in endometriotic lesions compared with endometrium[J].Biol Reprod,2001,65(4):1297-1303.
  • 9Collette T,Bellehumeur C,Kats R,et al.Evidence for an increased release of proteolytic activity by the eutopic endometrial tissue in women with endometriosis and for involvement of matrix metalloproteinase-9[J].Hum Reprod,2004,19(6):1257 -1264.
  • 10Bellehumeur C,Collette T,Maheux R,et al.Increased soluble interleukin-1 receptor type Ⅱ proteolysis in the endometrium of women with endometriosis[J].Hum Reprod,2005,20(5):1177-1184.

二级参考文献40

  • 1Kim YI, Park S, Jeoung DI, et al. Point mutations affecting the oligomeric structure of Nm23-H1 abrogates its inhibitory activity on colonization and invasion of prostate cancer cells. Biochem Biophys Res Commun, 2003, 307(2): 281-289.
  • 2Bruner KL, Matrisian LM, Rodgers WH, et al. Suppression of matrix metalloproteinases inhibits establishment of ectopic lesions by human endometrium in nude mice. J Clin Invest, 1997, 99( 12): 2851-2857.
  • 3Bullimore DW. Endometriosis is sustained by tumour necrosis factor-alpha. Med Hypotheses, 2003, 60( 1 ): 84-88.
  • 4Cox KE, Piva M, Sharpe-Timms KL. Differential regulation of matrix metalloproteinase-3 gene expression in endometriotic lesions compared with endometrium. Biol Reprod, 2001, 65(4): 1297-1303.
  • 5Ohbayashi H. Matrix metalloproteinases in lung diseases. Curr Protein Pept Sci, 2002, 3(4): 409-421.
  • 6Henriet P, Cornet PB, Lemoine P, et al. Circulating ovarian steroids and endometrial matrix metalloproteinases (MMPs). Ann N Y Acad Sci, 2002, 955(1): 119-138.
  • 7Chen SL, Wu YS, Shieh HY, et al. P53 is a regulator of the metastasis suppressor gene Nm23-H1. Mol Carcinog, 2003, 36 (4): 204-214.
  • 8Niitsu N, Honma Y, Iijima K, et al. Clinical significance of nm23-H1 proteins expressed on cell surface in non-Hodgkin's lymphoma. Leukemia, 2003, 17( 1 ): 196-202.
  • 9Otsuki Y, Tanaka M, Yoshii S, et al. Tumor metastasis suppressor nm23H1 regulates Rac1 GTPase by interaction with Tiam1. Proc Natl Acad Sci USA, 2001, 98(8): 4385-4390.
  • 10Campbell IG, Thomas EJ. Endometriosis: candidate genes. Hum Reprod Update, 2001, 7( 1 ): 15-20.

共引文献19

同被引文献22

  • 1黄海玲,韦叶生,黄海妮,解继胜,赵琼芝.雌激素受体-α基因多态性与子宫内膜异位症的相关性研究[J].生殖与避孕,2005,25(1):18-21. 被引量：8
  • 2BISCHOFF F,SIMPSON JL.Genetic basis of endometriosis[J].Ann N Y Acad Sci,2004,1034:284-299.
  • 3FRA¨NZEL J.B,VAN DUIJNHOVEN,IRENE D.Bezemer,et al.Polymorphisms in the Estrogen Receptor A Gene and Mammographic Density[J].Cancer Epidemiol Biomarkers Prev,2005,14(11):2655-2660.
  • 4HSIEH YY,WANG YK,CHANG CC,et al.Estrogen receptor alpha-351XbaI*G and -397 PvuII * C-related genotypes and alleles are associated with higher sescep-tibilities of endometriosis and leiomyoma[J].Mol Hum Reprod,2007b,13:117-122.
  • 5RENNER SP,STRICK R,OPPELT P,et al.Evaluation of clinical parameters and estrogen receptor alpha gene polymorphisms for patients with endometriosis[J].Reproduction,2006,131:153-161.
  • 6WANG Z,YOSHIDA S,NEGORO K,et al.Polymorphisms in the estrogen receptor beta gene but not estrogen receptor alpha gene affect the risk of developing endo-metriosis in a Japanese population[J].Fertil Steril,2004,81:1650-1656.
  • 7KITAWAKI J,OBAYASHI H,ISHIHARA H,et al.Oestrogen receptor-alpha gene poly-morphism is associated with endometriosis,adenomyosis and leiomyomata[J].Hum Reprod,2001,16:51-55.
  • 8GUO S-W.The association of endometriosis risk and genetic polymorphisms in-volving dioxin detoxification enzymes:a systematic review[J].Eur J Obstet Gynecol Repro Biol,2006b,124:134-143.
  • 9TEMPFER C.B,SIMONI M,DESTENAVES B,et al.Functional geneticpolymorphisms and female reproductive disorders:Part II-endometriosis[J].Human Reproduction Update,2009,15(1):97-118.
  • 10Cao X,Yang D,Song M,et al.The presence of endometrial cells in the peritoneal cavity enhances monocyte recruitment and induces inflammatory cytokines in mice:implications for endometriosis[J].Fertil Steril,2004,82(Suppl 3):999-1007.

引证文献2

二级引证文献1

中国病理生理杂志
2007年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部