摘要
目的体内研究慢性粒细胞白血病（CML）患者骨髓中BCR／ABL＋、Flk1＋CD34-细胞的白血病干细胞特性。方法取CML患者骨髓，淋巴细胞分离液分离单个核细胞，常规接种培养，免疫磁珠分选CD45-、GlyA-及CD34-的贴壁细胞，流式细胞术鉴定其免疫表型，将其输入经亚致死量照射的NOD／SCID小鼠体内，检测人源细胞的植入及分化情况。结果植入的CML患者骨髓源BCR／ABL＋、Flk1＋CD34-细胞在受体小鼠体内分化为白血病细胞，受体小鼠骨髓细胞中存在Bcr／Ab1融合基因阳性细胞。结论Ber／Ab1融合基因阳性Flk1＋CD34-细胞具有白血病干细胞特性。
Objective To study the leukemia stem cell characteristics of the Flk1＋CD34- cells carrying Bcr/Ab1 fusion gene from the bone marrow of CML patients in vivo. Methods Bone marrow was extracted from the Ph＋ patients with CML, mononuclear cells were separated by a Ficoll-Paque gradient centrifugation, throw off the suspended cells, using magnetic-activated cell separation （MACS） harvested the CD45-, GlyA-, and CD34- from the adherent cells, then identified the immunophenotype by fluorescence-activated cell sorter （FACS）, then transplanted the cells to the NOD/SCID mice, as well as detected the implantation and differentiation of the human origin cells, meanwhile observation the bone marrow hyperplasia of the receptor mice. Results The engrafted Flk1＋CD34- cells carrying Bcr/Ab1 fusion gene from the bone marrow of CML patients can differentiate to the leukemia cells, it can be detected the Bcr/Ab1 fusion gene positive cells from the bone marrow of receptor mice. Conclusion The Flk1＋CD34- cells carrying Bcr/Ab1 fusion gene have the characteristic of the leukemia stem cell.
出处
《国际输血及血液学杂志》
CAS
2007年第1期14-19,共6页
International Journal of Blood Transfusion and Hematology
