摘要
目的:探讨麻黄碱对缺血-再灌注损伤大鼠的神经保护机制.方法:体内部分:正常大鼠,分为4组,每组6只,分别腹腔注射不同剂量(2,4,8mg/kg)麻黄碱和等体积生理盐水,每3日1次,共4次,应用放射免疫组织化学方法检测大鼠血浆中血栓素B2和6酮前列腺素F1α浓度,并且计算两者比值.MCAO模型大鼠分组、给药剂量及方法同于正常组.体外部分:培养大鼠脑皮质微血管内皮细胞,分为麻黄碱给药组(培养基麻黄碱的浓度分别为2,4,8mg/L)和对照组(培养基无麻黄碱)培养2h后检测6酮前列腺素F1α浓度.结果:体内部分:①正常大鼠:麻黄碱8mg/kg组TXB2,6-Keto-PGF1α和T/P低于正常对照组(P<0.05).②MCAO模型大鼠:各给药组TXB2和T/P均低于对照组(P<0.05).③MCAO模型对照组TXB2,6-Keto-PGF1α和T/P高于正常对照组(P<0.05).体外试验:麻黄碱各浓度组6-Keto-PGF1α与对照组的差别无统计学意义(P>0.05).结论:麻黄碱对大鼠血浆TXA2-PGI2平衡的影响可能与其对缺血-再灌注损伤大鼠的神经保护性作用有关.
AIM: To investigate the protective effects of ephed- rine on the rats with cerebral ischemia-reperfusion injury. METHODS: In vivo: Normal rats were randomly divided into 4 groups and injected respectively with ephedrine (2, 4, 8 mg/kg) and equal volume normal saline, once every 3 d, totally for 4 times. The plasma concentrations of thromboxane B2 ( TXB: ) and 6-keto-PGF1α were detected by radio-immunohistochemistry. The ratio of TXB2 to 6-keto-PGF1α (T/P) was calculated. The subgrouping, administrated doses and manner in middle cerbral artery occlusion (MCAO) group are same as the normal control group; In vitro: The brain microvascular endothelial cells were cultured and divided into 3 ephedrine groups (2, 4 and 8 mg/L) and 1 control group (no ephedrine). The plasma concentration of 6- keto-PGF1α was detected after 2 h. RESULTS: In vivo: In the normal rats, T/P, the plasma concentrations of TXB2 and 6-Keto- PGF1α in the groups treated with 8mg/kg ephedrine were significandy lower than those in the control group ( P 〈 0.05 ). In the MCAO rats, compared to the control group, the TXB2 concentration and T/P in all treated groups were significantly lower (P 〈 0.05) ; In vitro : The concentration of 6-Keto-PGF1α in each group was not different statistically (P 〉 0.05). CONCLUSION: The protective effects of ephedrine on the MCAO rats may be related to balancing the ratio of TXA2/PG1α.
出处
《第四军医大学学报》
北大核心
2007年第3期236-239,共4页
Journal of the Fourth Military Medical University
基金
重庆市卫生局中医药科研项目(2003-B-38)
关键词
血栓素
前列腺素
麻黄碱
脑缺血
大鼠
thromboxane
prostaglandin
ephedrine
cerebral ischemia
rat