钙调素阻滞剂三氟拉嗪对原代肺癌细胞作用的研究

Effects of trifluperazine, a calmodulin antagonist, on the primany lung cancer cells

应用细胞培养技术对２８例手术切除标本行原代肺癌细胞培养，并测定了钙调素（ＣａＭ）阻滞剂三氟拉嗪对原代肺癌细胞的作用。结果显示：三氟拉嗪能抑制肺癌细胞进入增殖周期，使细胞阻滞于Ｇ０／Ｇ１期。在长春新碱（ＶＣＲ）、阿霉素（ＡＤＲ）和足叶乙甙（ＶＰ１６）各药物组加入三氟拉嗪后能使癌细胞活性（Ａ值）明显降低（Ｐ＜０．０１或Ｐ＜０．０５），单用三氟拉嗪组亦能使癌细胞活性降低，但无统计学意义（Ｐ＞０．０５）。结果表明：三氟拉嗪对原代肺癌细胞增殖有一定抑制作用，能明显增强ＶＣＲ、ＡＤＲ和ＶＰ１６的体外抗癌活性。

After 28 speimens of the primary human lung cancer cells obtained by operation were cultured,the effects of trifluperatine (TFP), a calmodulin (CaM) antagonist, on the primary lung cancer cells were investigated. It was found that TFP inhibited the proliferation of cancer cells and thus the percentage of cells at G 0/G 1 phase was increased significantly. The activity of cancer cells was significantly weakened when TFP was added into the VCR,ADR and VP16 treating groups ( P <0.01 or P <0.05). These findings suggest that TFP can exert certain degree of inhibitory effects on the proliferation of primary lung cancer cells and enchance the anticancer effects of VCR, ADR and VP16 in vitro. The latter may be due to the inhibitory effects of TFP on CaM and P gp.