微球法制备聚乳酸/β-磷酸三钙组织工程支架的研究

Study of Microsphere-based Tissue Engineered Scaffold Made by Poly(lactic acid)/β-tricalcium Phosphate

本文采用一种新颖的微球法制备了聚乳酸/β-磷酸三钙组织工程支架。并通过孔隙率的测定、电镜及抗压模量的测试来研究β-磷酸三钙的加入量以及搅拌时间对多孔支架的结构以及力学性能的影响。研究发现,对于同一组分的材料随着搅拌时间的增长,多孔支架的孔隙率有增大的趋势,同时多孔支架的抗压模量不断增大。随着β-磷酸三钙加入量的增加,多孔支架的孔隙率有先增大后减小的趋势。当在2 g聚乳酸中加入0.2 gβ-磷酸三钙时孔隙率达最大。加入β-磷酸三钙后的多孔支架都不同程度比同样条件下纯聚乳酸多孔支架的抗压模量有所降低,但当加入0.2 gβ-磷酸三钙后,以300转/分钟的速度搅拌2 h时,所制得的多孔支架的抗压模量与纯聚乳酸多孔支架的抗压模量基本相当。在2 g聚乳酸中加入0.2 gβ-磷酸三钙,以300转/分钟的速度搅拌2 h时,材料具有较高的孔隙率(55.5%),同时也具有较高的抗压模量275 MPa,是较好的反应条件。

In the paper, a novel method was used to prepare Poly（lactic acid）/β-tricalcium phosphate microsphere-based tissue engineered scaffold. It was found that the porosity of scaffolds with the same component had an increased trend with the prolonging of the stirring time. The compressive modulus of scaffolds was also increased with the prolonging of the stirring time. With the increase of the amount of β-tricalcium phosphate, the porosity of scaffolds was increasd at first and then decreased. Compared to pure PLA scaffold, the compressive modulus of PLA scaffolds with β-tricalcium phosphate was decreased to some extent. However, the compressive modulus of PLA Scaffolds with 0.2 g β-tricalcium phosphate and stirred for 2 h（300 rpm） was almost same to the compressive modulus of pure PLA scaffold. At the same time, the porosity of PLA scaffolds with 0.2 g β-tricalcium phosphate and stirred for 2 h （300 rpm） was 55.5 % which was higher than the porosity of pure PLA scaffold（20～40%）. So, the PLA scaffolds with a higher strength and porosity could be obtained under the optimum conditions （i.e., 2.0 g of PLA, 0.2 g of β-tricalcium phosphate, 300 rpm, stirring time for 2 h ）.