高效液相色谱法测定桑叶中1-脱氧野尻霉素的含量

Determination of DNJ in Morus alba L.leaves by HPLC

目的采用HPLC—ELSD法测定不同产地桑叶中1-脱氧野尻霉素的含量。方法色谱柱为TSKgel Amide-80（4．6mm×250mm，5μm）；流动相为乙腈-水（70：30），水中含有10mmol/L醋酸铵；流速1．0ml／min。结果线性范围为2.11～10．55μg，r=0．9991。平均回收率为95．98％（n=5），RSD=1．98％。结论本方法快速、简便、重现性好，可用于桑叶及相关样品中DNJ的定量分析。

Objective To determine the content of 1 - deoxynojirimycin in Morus alba L.leaves from different areas using HPLC - ELSD.Methods A HPLC method was established using TSKgel Amide- 80（4.6 × 250 mm,5μm）with acetonitrile- water （70： 30） as mobile phase and the water containing 10 mmol/L ammonium acetate. The flow rate was 1.0 ml/min.Results The calibration curve was linear in the range of 2.11～10. 55μg,r=0.9991. The average recovery was 95.98%（ n=5）, RSD=1.98%.Conclusions The method is rapid, simple, stable and can be used for the determination of 1 - deoxynojirimycin in mulberry leaves and other related products.