实验性大鼠脑震荡后Bcl-2的表达

Bcl-2 Expression Following the Brain Concussion in Rats

目的观察实验性大鼠脑震荡后Bcl-2表达的变化规律。方法应用免疫组织化学方法检测大鼠脑震荡后不同时间大脑皮层、脑干和小脑等脑区内Bcl-2表达的变化规律进行研究。结果对照组大鼠未见Bcl-2蛋白的表达。脑震荡组损伤后1h组可在神经细胞内观察到少量Bcl-2蛋白的表达,4d达到高峰,8d和16d,Bcl-2蛋白表达随后逐渐下降。结论Bcl-2的检测可能成为脑震荡的诊断和脑损伤时间推断的一个敏感指标。

Objective To evaluate the expression of Bcl-2 protein after brain concussion. Methods Expression levels of Bcl-2 protein in cortex, pontine and cerebellum of rats were investigated using immunohistochemistry. Results There was no expression of Bcl-2 protein in control group seen. The expression of Bcl-2 protein in brain concussion groups was detected at lhour, and the expression level reached its peak 4 days after the concussion and then declined gradually. Conclusion Our findings suggest that the detection of Bcl-2 protein could be an indicator for diagnosis of brain concussion and for estimation of the post injury time interval.