低温保存过程中老鼠成骨细胞胞内冰的研究

Intracellular Ice Formation of Mouse Osteoblasts During Cryopreservation

低温保存过程中,胞内冰是造成细胞损伤的主要因素之一,运用实验与模型相结合的手段,研究细胞胞内冰的生成规律,可以为细胞低温保存方案的设计提供可靠的依据。应用低温显微镜系统,结合胞内冰生成模型,系统地研究了老鼠成骨细胞在低温保存过程中的胞内冰生成规律。在首先获得水渗透参数及胞内冰成核参数的基础上,应用数学模型预测了多种降温速率下老鼠成骨细胞的胞内冰生成率,结果表明,实验数据与模型计算能够很好的吻合。

Intracellular ice formation （IIF） is one of the factors that causes cell damage during cryopreservation process. The study of IIF will provide fundamental information for the development of appropriate cryopreservation protocols. The possibility of IIF at various cooling rates for mouse osteoblasts was studied by using a cryomicoscopy system. The water transportation and ice nucleation parameters were used in the coupled water transport/ice nucleation model to predict the probability of IIF over a wide range of cooling rates. The calculated probability of IIF was in good agreement with the experimental data, suggesting the validity of the predicted parameters.