Ca^（2＋）对罗非鱼EOG影响及其解毒可行性探讨

Effect of Ca￣(2+) on the EOG of Tilapia sp. and Exploration of Detoxifying Feasibility

将四种浓度的Ｃａ２＋溶液和含４ｍｍｏｌ／Ｌ简Ｃａ２＋的不同浓度的Ｃｕ２＋、Ｚｎ２＋及其混合液，分别灌注鱼的嗅囊，研究它们对罗非鱼嗅电图（下简ＥＯＧ）的影响，并初步探讨Ｃａ２＋作为解毒剂的可行性及机制．结果表明：１）在一定浓度Ｃａ２＋范围内，ＥＯＧ振幅随Ｃａ２＋浓度的增加而增大，超此范围，则随浓度的增加而减小；２）Ｃａ２＋能使Ｃｕ２＋、Ｚｎ２＋中毒鱼的ＥＯＧ反应振幅接近正常值，反映其具一定解毒作用，效果顺序为Ｃｕ２＋＞Ｚｎ２＋；３）Ｃａ２＋对Ｃｕ２＋、Ｚｎ２＋混合液也具一定解毒作用．

The effects of calcium solutions are studied by recording the receptor potential, electro-olfactogram (EOG) form the olfactory epitheliu of Tilapia sp.. In series of experiments, the olfactory organ is irrigated with these solutions, including four Ca2+ concentrations,the various mixture containing 4 mmol/L Ca2+ and the different concentrations of either Cu2+ or Zn2+or both of Cu2+ +Zn2+. The feasibility and mechanism of Ca2+ as detoxifying substance are discussed. These results indicate that: 1 ) In the certain concentration range, the amplitudes of EOG increase with increasing concentration of Ca2+,and beyond the range,decrease with Ca2+ increasing; 2)The amplitude of EOG close to the normal value when the poisoned fish was treated with Ca2+,showing that Ca2+has detoxifying effects on heavy metal ions (Cu2+ .Zn2+ ) with the order being Cu2+ >Zn2+. 3) Ca2+ has also a certain detoxifying effects on the mixture solutions.