石斛属植物组织培养及遗传转化研究进展

Research Progress in Dendrobium Tissue Culture and Genetic Transformation

石斛作为一种集药用和观赏于一体的珍稀植物,近年来其组培快繁和基因工程研究取得了比较大的进展。综述了石斛属植物组织培养外植体、培养基、增殖与分化、遗传转化方法、靶材料、选择标记基因和报告基因等方面的研究进展,为石斛的开发和利用提供科学依据。

The tissue culture, rapid propagation and genetic eigineering of Dendrobium plant have been advanced obviously in recent years. In the.present paper the progress in its tissue culture including explants, media and cultured methods were outlined, the advance in its genetic transformation involving in transformation metheds, target tissue, selection of genes and report genes were reviewed. The prospect and problem in the tissue culture and genetic engineering of the Dendrobium were also discussed.